FIGURE 3.

Altered cell cycle properties of HSCs from IKK2^CA mice. (A) Representative FACS plots (left) and average frequencies (right) of AnnexinV⁺ cells in LSK fractions in the BM of 6 weeks old IKK2^CA and control mice. Data are pool of 5 mice per group. (B) Representative FACS plots (left) and average frequencies (right) of BrdU⁺ cells in LSK cells and LT-HSCs in the BM of 6 weeks old IKK2^CA and control mice. Data are representative of two independent experiments with 3 mice per group. (C) Representative FACS plots (left) indicating frequencies of HSPCs in G0 (Pyronin Y^- and Hoechst^-), G1 (Pyronin Y⁺ and Hoechst^-) and G2/S (Pyronin Y⁺ and Hoechst⁺) phase of cell cycle in the BM of 6 weeks old IKK2^CA and control mice. Shown are average frequencies of HSPCs in G0 phase of cell cycle in the BM of 6 weeks old IKK2^CA and control mice (right). Data are representative of two independent experiments with 3 mice per group. Representative FACS plots (left) and average frequencies (right) of side population (SP) fraction in Sca1⁺c-Kit⁺ (SK) cells (D) and CD48^-CD150⁺Sca1⁺c-Kit⁺ cells (E) in the BM of 6 weeks old IKK2^CA and control mice. Data are representative of two independent experiments with 3 mice per group. All data represent mean ± SEM. Two-tailed Student’s t-tests were used to assess statistical significance (^∗P < 0.05, ^∗∗P < 0.01).