FIGURE 7.

Increased NF-κB binding to Junb enhancer region in hematopoietic cells from IKK2^CA mice. (A) NF-κB target genes that are upregulated in IKK2^CA LT-HSCs. (B) Diagrammatic representation of the Junb gene indicating the presence of three NF-κB binding sites in its enhancer region. (C) NF-κB binding sites in enhancer region of the Junb gene of indicated species. (D) Chromatin immunoprecipitation (ChIP) analysis of NF-κB (p65) binding to Junb promoter in the hematopoietic cells of 6 weeks old IKK2^CA and control mice. Shown are the Real Time PCR data of p65 immunoprecipitates, that were normalized to IgG control immunoprecipitates. Data are representative of two independent experiments. All data represent mean ± SEM. Two-tailed Student’s t-tests were used to assess statistical significance (^∗P < 0.05, ^∗∗P < 0.01).