FIGURE 1.

Analysis of gene expression in transgenic cassava lines. (A) Immunoblot detection of GWD proteins (G, wild-type StGWD protein; R, redox insensitive version) in soluble protein extracts from cassava storage root (5 μg protein loaded). Wild-type (WT) control and 3 technical replicates of pooled material (from 3 or more individuals of each transgenic line) were analyzed. Actin (loading control) was detected on the same membrane. (B) Growth comparison of representative G- and R-lines. (C) Growth comparison of representative LSF2 RNAi lines (L-lines) and SEX4 RNAi lines (S-lines). Red arrows indicate shoot branching. Bar = 15 cm. (D) Immunoblot detection of the MeSEX4 protein in soluble protein extracts (5 μg protein loaded). Wild-type control and 2–4 technical replicates of pooled material (from 3 or more individuals of each transgenic line) were analyzed. Actin was used as a loading control. (E) Expression of the LSF2 gene in storage roots relative to the PP2A reference gene determined by RT-PCR. Mean values (±SD) of three replicate analyses of pooled material (from 3 or more individuals of each transgenic line). These experiments were repeated three times with similar results with independently grown populations of plants.