Table 7.
Growth and glutarate production data of recombinant C. glutamicum strains that lack glutamate dehydrogenase.
| GRLys1ΔsugRΔldhAΔsnaAΔcgmAΔgdh+ | Growth rate [h-1] |
Biomass [g L-1] |
Glutarate titer [g L-1] |
Glutarate yield [g g-1] |
Glutarate vol. prod. [g L-1 h-1] |
|---|---|---|---|---|---|
| (pVWEx1)(pEKEx3)(pECXT99A) | 0.03 ± 0.00 | 3.7 ± 0.2 | – | – | – |
| (pVWEx1-ldcC)(pEKEx3-patDA)(pECXT99A) | 0.05 ± 0.00 | 4.3 ± 0.4 | 1.7 ± 0.0 | 0.04 ± 0.00 | 0.03 ± 0.00 |
| (pVWEx1-ldcC)(pEKEx3-patDA)(pECXT99A-gabTDCglu) | 0.05 ± 0.00 | 4.1 ± 0.3 | 3.3 ± 0.1 | 0.08 ± 0.00 | 0.05 ± 0.00 |
| (pVWEx1-ldcC)(pEKEx3-patDA)(pECXT99A-gabTDPpu) | 0.05 ± 0.01 | 4.3 ± 0.2 | 5.0 ± 0.1 | 0.12 ± 0.01 | 0.08 ± 0.00 |
| (pVWEx1-ldcC)(pEKEx3-patDA)(pECXT99A-gabTDPsyr) | 0.06 ± 0.00 | 4.0 ± 0.4 | 4.7 ± 0.2 | 0.12 ± 0.00 | 0.08 ± 0.00 |
| (pVWEx1-ldcC)(pEKEx3-patDA)(pECXT99A-gabTDPstu) | 0.07 ± 0.00 | 4.6 ± 0.3 | 5.2 ± 0.1 | 0.13 ± 0.00 | 0.09 ± 0.00 |
Samples were harvested and analyzed after glucose depletion. Values represent means and standard deviations of triplicate cultivations.