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. 2018 Oct 30;9:1569. doi: 10.3389/fpls.2018.01569

FIGURE 4.

FIGURE 4

Jasmonate content in individual Arabidopsis leaves after wounding, sampled with the cut-and-freeze-method from young to old leaves. Mean levels of OPDA (A), JA (B), and JA-Ile (C) ±SE in individual leaves after wounding leaf 8 with MecWorm for 1 h. Untreated plants were used as control. Legend for color code see (A). Leaves were collected with the cut-and-freeze-method from leaf 13 (young) to leaf 5 (old) as indicated by the arrow in (A). The experiment was repeated three times independently (n ≥ 13). Statistically significant differences between phytohormone content of treated and untreated plants within the leaves were determined by unpaired two-sample Wilcoxon test. Asterisk indicates significance (∗∗∗P < 0.001). P-values are FDR corrected.