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. 2018 Oct 30;9:1569. doi: 10.3389/fpls.2018.01569

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Copyright © 2018 Heyer, Reichelt and Mithöfer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phytohormone content of untreated Arabidopsis leaves using the freeze-and-cut-method. Bars represent mean levels of OPDA (A), JA (B), JA-Ile (C), SA (D), and ABA (E) ± SE in individual leaves of untreated Arabidopsis plants. Individual leaves were collected with the freeze-and-cut-method from leaf 13 (young) to leaf 5 (old). Sampling direction is indicated by an arrow above the figures. The experiment was repeated six times independently (n ≥ 19). Red lines indicate the mean values of untreated plants collected with the cut-and-freeze-method in the same sampling direction (see Figures 4, 5F,H).