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. 2018 Oct 30;9:1569. doi: 10.3389/fpls.2018.01569

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Copyright © 2018 Heyer, Reichelt and Mithöfer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Systemic pattern of phytohormones in individual leaves of the Arabidopsis rosette after wounding using the freeze-and-cut-method for sampling. Mean levels of SA (A), ABA (B), OPDA (C), JA (D), and JA-Ile (E) ± SE in individual leaves after wounding leaf 8 with MecWorm for 1 h.(Untreated plants were used as control. Legend for color code see (A). Leaves were collected with the freeze-and-cut-method from leaf 13 (young) to leaf 5 (old) as indicated by the arrow above the figures. The experiment was repeated five times independently (n ≥ 16). Statistically significant differences between phytohormone content of treated and untreated plants within the leaves were determined by unpaired two-sample Wilcoxon test. Asterisk indicates significance (^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001). P-values are FDR corrected.)