Skip to main content
. 2018 Oct 29;20:321–333. doi: 10.1016/j.redox.2018.10.009

Fig. 4.

Fig. 4

The chimeric factor VBH135 functions as a dominant and constitutive transcription activator. (A) Rat UCP-1 gene promoter driven reporter, rUCP-1-luc, was co-transfected with PPARγ, PGC-1α, and Bhlhe40 (full length or 1–135) expression vectors into C2C12 myoblasts in the presence of troglitazone (1 μM). * *: p < 0.01 vs. promoter only; # and ##: p < 0.05 and p < 0.01, respectively, vs. PPARγ activated promoter activity. (B) Schematic representations of the molecular organization of VBH135, a chimeric fusion protein of large T antigen nuclear localization signal, VP16 activation domain (414−490), and Bhlhe40 N-terminal 1–135 amino acids. (C) Gal-PGC-1α expression vector was co-transfected with MH100-luc reporter, with or without Bhlhe40 (FL or 1–135) into C2C12 myoblasts. * and * *: p < 0.05 and p < 0.01 vs. Gal-DBD only. # and ##: p < 0.05 and p < 0.01 vs. Gal-PGC-1α-(FL). $: p < 0.05 vs Gal-PGC-1α-(1−128). (D)M-cadherin-luc reporter was co-transfected with or without Bhlhe40, VBH135 or VBH135m expression vectors into C2C12 myoblasts. All transfections were initiated when myoblasts were at about 70% confluence and lasted for overnight till cells become confluent. Then, cells were kept in differentiation medium to induce myotube formation for 72 h before harvested for determining luciferase activity.