Fig. 6.

The effects of VBH135 or VBH135m over-expression on mitochondrion and peroxisome activity. The levels of ROS, mtDNA, and Mitotracker stain intensity of C2C12-VBH135 and -VBH135m cells at CMB and MT stages in the presence and absence of Dox are shown in (A), (B), and (C), respectively. The MITO efficiency (D) was calculated by Mitoctracker/mtDNA and compared with cells treated with Dox (+). (E) Images of C2C12-VBH135 and -VBH135m myoblasts transfected with RFP-PTS1 expression vector and counterstained with DAPI. The average number of peroxisomes/cell (F) and the distribution (%) of cells with different numbers of peroxisome/cell (G & H) of peroxisome number/cell are calculated 48 h after transfection. * and * *: p < 0.05 and p < 0.01, respectively, vs. Dox (+) cells.