Fig. 8.

The genes targeted by Bhlhe40 and VBH135. The genes expression patterns of C2C12-VBH135 and C2C12-Bhlhe40 myotubes and C2C12-shBhlhe40 myoblasts were examined with qRT-PCR. The relative levels of genes involved in peroxisome or mitochondrial biogenesis and functions are shown in (A) and (B) respectively. The results of genes involved in ROS scavenging are shown in (C). Binding of Bhlhe40 to target gene promoters was examined in C2C12-Bhlhe40 myotubes (Dox (-)) by ChIP assay (D). PGC-1α was knockdowned in C2C12-VBH135(E) and -Bhlhe40(F) myotubes to reveal PGC-1α-dependent gene regulation. * and * *: p < 0.05 and p < 0.01, respectively, vs. Dox (+) or control cells. ^# and ^##: p < 0.05 and p < 0.01 vs. control ShRNA treated Dox (+)/(-) in (E) and (F).