REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
MAX Efficiency DH10Bac™	Thermo Fisher	Cat# 10361012
Biological Samples
Mouse liver microsomes	Sigma	Cat# M9441
Human liver microsomes	Sigma	Cat# M 0317
Chemicals, Peptides, and Recombinant Proteins
Tunicamycin	Sigma-Aldrich	Cat# T7765; CAS: 11089-65-9
uridine diphosphate n-acetyl [1-14C] D-glucosamine	American Radiolabeled Chemicals	Cat# ARC 0151
Rifampicin	Sigma-Aldrich	Cat# R3501
Uridine 5′-diphospho-N-acetylglucosamine sodium salt	Sigma-Aldrich	Cat# U4375
C95-Dolichyl-MPDA	Larodan	Cat# 67-1095
H. sapiens DPAGT1 (1-408, N-terminal 6 x HIS tag, TEV cleavage site)	This work	N/A
H. sapiens DPAGT1 (1-408, Val264Gly, N-terminal 6 x HIS tag, TEV cleavage site)	This work	N/A
Critical Commercial Assays
MemGold2 HT-96 screen	Molecular Dimensions	Cat# MD1-64
Deposited Data
H. sapiens DPAGT1 (WT)	This work	PDB: 6FM9
H. sapiens DPAGT1 (Val264Gly)	This work	PDB: 5LEV
H. sapiens DPAGT1 (Val264Gly) + UDP-GlcNAc + Mg2+	This work	PDB: 6FWZ
H. sapiens DPAGT1 (Val264Gly) + Tunicamycin	This work	PDB: 5O5E
Experimental Models: Cell Lines
Spodoptera frugiperda (Sf9) insect cells	Thermo Fisher	Cat# 11496015; RRID:CVCL_0549
HepG2	ATCC	Cat# ATCC HB-8065
HEK293	ATCC	Cat# ATCC CRL-1573; RRID:CVCL_0045
Raji	ATCC	Cat# ATCC CCL-86; RRID:CVCL_0511
J774A.1	ATCC	Cat# ATCC TIB-67; RRID:CVCL_0358
Experimental Models: Organisms/Strains
Streptomyces chartreusis	DSMZ	Cat# 41447
Bacillus subtilis EC1524	John Innes Centre	N/A
Micrococcus luteus	John Innes Centre	N/A
Bacillus cereus	DSMZ	Cat# 345
Escherichia coli	Thermo Fisher	Cat# ATCC 25922
Staphylococcus aureus	Thermo Fisher	Cat# ATCC 29219
Pseudomonas aeruginosa	Thermo Fisher	Cat# ATCC 27853
Mycobacterium tuberculosis H37Rv	ATCC	Cat# ATCC27294
C57Bl/6 mice	Taconic	B6
Oligonucleotides
Oligonucleotides	See table S8	B6
Recombinant DNA
pFB-LIC-Bse	This paper	N/A
DPAGT1 gene	Source BioScience LifeSciences	IMAGE:2821845
pMSP12::GFP	Addgene	Cat# 30167
Software and Algorithms
Prism 7	GraphPad	www.graphpad.com
X-ray Detector Software (XDS)	(Kabsch, 2010)	http://xds.mpimf-heidelberg.mpg.de/
CCP4 Suite	(Winn et al., 2011)	http://www.ccp4.ac.uk/
PHYRE2 webserver	(Kelley et al., 2015)	http://www.sbg.bio.ic.ac.uk/phyre2
MR_ROSETTA (phenix.mr_rosetta)	(Terwilliger et al., 2012)	https://www.phenix-online.org/documentation/reference/mr_rosetta.html
PHENIX	(Adams et al., 2010)	https://www.phenix-online.org/
BUSTER (v.2.10.2 & v2.10.3)	(Bricogne et al., 2017)	https://www.globalphasing.com/buster/
COOT	(Emsley et al., 2010)	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PyMOL	Schrödinger, LLC	https://www.pymol.org/
MOLPROBITY	(Chen et al., 2010)	http://molprobity.biochem.duke.edu/
STARANISO	Global Phasing Ltd	http://staraniso.globalphasing.org
PDB2PQR	(Dolinsky et al., 2004)	http://nbcr-222.ucsd.edu/pdb2pqr_2.0.0/
UCSF Chimera	(Pettersen et al., 2004)	https://www.cgl.ucsf.edu/chimera/
Mass spectrometry software Qual Browser	Thermo Fisher	Xcalibur 2.2