Table 1.
Flow cytometry panel design with 8 colour fluorochromes for immune subset analysis includes lymphocyte (T & B), monocytes, macrophages and dendritic cells in resected tumour samples from HNSCC patients and matched peripheral blood samples.
| Cell Type | Tube | BV421 | V500/BV510 | FITC/BB 515 | PE/PE-CF594 | PerCp-Cy5.5 | PE-Cy7 | APC/Alexa647 | APC-H7 |
|---|---|---|---|---|---|---|---|---|---|
| T cell | T1 | CD45RA | CD3 | CD8 | CCR7 | CD4 | CD45RO | CD38 | HLA-DR |
| T2 | Perforin | CD3 | CD8 | Granzyme B | CD4 | CD16 + CD56 | CD103 | - | |
| T3 | GARP | CD3 | CD8 | LAP | CD4 | CD278 | - | ||
| T4 | TIM-3 | CD3 | CTLA4 | CD4 | PD-1 | PDL-1 | - | ||
| Treg | T1 | CD3 | CD25 | FoxP3 | CD4 | CD127 | - | ||
| B cell | T1 | CD24 | CD138 | CD27 | CD10 | CD19 | IgD | CD38 | CD20 |
| MDSC, Monocyte, DC | T1 | CD200 | CD3 | CD16 | CD1c | CD11c | CD14 | CD169 | HLA-DR |
| T2 | CD141 | - | CD15 | - | CD11c | - | CD123 | HLA-DR | |
| Macro-phage | T1 | CD40 | HLA-DR | CD206 | CD81 | - | CD33 | CD192 | CD11b |
| T2 | CD141 | CD86 | CD163 | CD80 | - | PD-1 | PDL-1 | CD11b |