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Characterization of the envZ and ompR deletion in wild-type and opgG background. (A) Schematic of the envZ-ompR locus in the wild-type strain and genetic organization of the mutant strains. (B) Expression of envZ and ompR was analyzed by qPCR. Bacteria were grown at 170, 330, 500, and 700 mOsM. Relative gene expression was calculated using ipxC as a reference (Hommais et al., 2011). Data represent mean ± standard deviation of three independent experiments.
