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. 2018 Oct 30;9:2459. doi: 10.3389/fmicb.2018.02459

FIGURE 3.

FIGURE 3

Effect of EnvZ-OmpR on motility in wild-type and opgG background. (A) Motility of wild-type, opgG, envZ, envZ opgG, ompR, and ompR opgG strains. Motility was measured in M63 semisolid plates. Swim diameters were measured after 48 h of incubation at 30°C. (B) Schematic of the regulatory cascade of motility. FlhDC, a master regulator and a class I promoter, modulates gene expression with a class II promoter (e.g., fliA). In return, the products of those genes regulate the genes with a class III promoter (e.g., fliC). (C,D) Expression of (C) flhD and (D) fliC in wild-type, opgG, envZ, envZ opgG, ompR, and ompR opgG strains. Bacteria were grown at 170, 330, 500, and 700 mOsM. The expression of (C) flhD, (D) fliC was analyzed by qPCR. Relative gene expression was calculated using ipxC as a reference (Hommais et al., 2011). Data represent mean ± standard deviation of 10 independent experiments. An asterisk indicates a significant difference with ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.01, and ∗p < 0.05.