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. 2018 Oct 30;9:2439. doi: 10.3389/fimmu.2018.02439

Figure 5.

Figure 5

CTT3H antigen-specific T cells in spleens induced by BCG prime-CTT3H-based boosters before exposure (n = 6). The immunization protocol was described in Figure 2D. At the eighteenth week, splenocytes from heterologous boosters with PBS, CTT3H-DMT, pCTT3H-DMT, rAdCTT3H, and BCG vaccinated mice were collected and counted. 2.5 × 106 cells were seeded in each well of a 24-well plate and stimulated with CTT3H (10 μg/mL) as described in detail in the section of Materials and Methods. The absolute number of CTT3H-specific IFN-γ+ (or IL-2 +) CD4 + (or CD8 +) T cells, IFN-γ+ CD4 + (or CD8 +) TEM cells, and IL-2+ CD4 + (or CD8 +) TCM cells from spleens were detected by flow cytometry. Results are shown as mean ± SD (n = 6). (A,B) Flow cytometry analysis strategy, (C) comparing BCG prime and BCG repeated boost strategy, (D,F) comparing BCG prime, subunit vaccine prime, and BCG prime and boost with subunit vaccine including protein vaccine (D), DNA vaccine (E), and recombinant adenovirus vaccine (F).