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Immunohistochemical analysis of heat shock proteins (HSPs) in chicken heart tissue (1 bar = 10 μm). After rosemary treatment, crystallin alpha B (CRYAB) and HSP70 showed higher staining intensity in the no heat stress (HS) group and in the 10 h heat stress group. However, HSP27 showed higher staining intensity before and after HS, except in the 10 h rosemary + heat group, in which the staining intensity was lower than in the control.
