Fig 3.

PRDX6 promotes the proliferation and colony formation of ESCC cells. (A) Eca-109 and TE-1 cells were infected with Ad-RFP or Ad-PRDX6. Cells were seeded in a 96- well plate and incubated for 48 h. Cell proliferation was measured by an MTT assay. The left panel shows the images of cells after MTT staining. (B) EdU assay of Eca-109 cell proliferation. Representative images of EdU positive cells from an EdU incorporation assay. The images were acquired using a fluorescence microscope under the same conditions. (C) Eca-109 and TE-1 cells were mock infected or infected with indicated lentiviruses. Cell proliferation was measured by an MTT assay. (D) Eca-109 cells were infected with lentiviruses targeting PRDX6. Cell proliferation was measured by an EdU assay. The effect of PRDX6 silencing on colony formation efficiency and colony size in (E) Eca-109 and (F) TE-1 cells. The left panel shows the representative colonies, and the right panel shows the data analysis. Relative colony size in each group was quantified by Image J software. Data are presented as mean ± SEM and were normalized to the control cells, * P < 0.05; ** P < 0.01.