Fig 5.

PRDX6 modulates the radiosensitivity of ESCC cells. (A) The effect of PRDX6 overexpression on ROS levels of Eca-109 cells. Cells infected with Ad-RFP or Ad-PRDX6. Fluorescent signals, reflecting the concentration of ROS, were measured by a fluorescence microscope under the same conditions. (B) Eca-109 cells were infected with indicated lentivirus. ROS levels were measured as mentioned above. (C) The effect of PRDX6 on cell apoptosis in Eca-109 cells. Cells were infected with Ad-PRDX6 or Ad-RFP then exposed to 8 Gy of irradiation. (D) Cells were infected with shRNA-NC or sh-PRDX6-3 lentivirus followed by 8 Gy irradiation. Data are presented as mean ± SEM and were normalized to the control cells, * P < 0.05; ** P < 0.01.