Fig 6.

PRDX6 and Erk1/2 pathway are mutually regulated. (A) Eca-109 cells were mock infected or infected with Ad-RFP or Ad-PRDX6. The expression of p-Erk1/2 and Erk1/2 were subjected to Western blotting. Relative expression of p-Erk1/2 in each group was quantified by Image J software. (B) Eca-109 cells were mock infected or infected with lentivirus expressing shRNA-NC, sh-PRDX6-2, sh-PRDX6-3. The expression of p-Erk1/2 and Erk1/2 were subjected to Western blotting. (C) Eca-109 cells were incubated with at concentrations of 0.1, 1.0, 5.0 or 10 μM PD0325901 (Erk1/2 pathway inhibitor). The expression of p-Erk1/2 and Erk1/2 were detected by Western blotting. (D) After treatment with various concentration of PD0325901, the expression of PRDX6 was measured by Western blotting in Eca-109 cells. Data are presented as mean ± SEM and were normalized to the control cells, * P < 0.05; ** P < 0.01.