Fig 7.
PRDX6 promoted esophageal cancer growth in vivo. Each group of mice was composed of five nude mice. Cells were infected with indicated virus. Bal b/c nude mice were subcutaneously grafted with 1 × 106 Eca-109 cells. Tumor volumes were measured every 3 days for 27 days, as described in the Materials and Methods. (A) Verification the overexpression of PRDX6 by Ad-PRDX6 in vivo. Western blotting showing the expression of PRDX6 in each group (Ad-RFP and Ad-PRDX6). Relative expression of PRDX6 of each group was quantified by Image J. (B) Body weight of nude mice of each group. (C) Tumor volume growth curve of each group. (D) Representative tumors from the mice of each group at the end time-point. (E) Immunohistochemical staining for PRDX6 and Ki67 in tumors from each group. (F) Verification the silencing of PRDX6 by lentiviruses in vivo. Western blotting showing the expression of PRDX6 in each group. (G) Body weight of nude mice of each group. (H) Tumor volume growth curve of each group. (I) Representative tumors from the mice of each group at the end time-point. (J) Immunohistochemical staining for PRDX6 and Ki67 in xenografts from each group.