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. 2018 Oct 10;9(21):3962–3970. doi: 10.7150/jca.25909

Fig 1.

Fig 1

Effects of Oxa to Bel-7404 and SMMC-7721 cells on cell apoptosis and cell cycle. (A) Cell viability was measured by CCK8 assay and 50% inhibitive concentration (IC50) of Oxa was calculated also. (B-D) Bel-7404 and SMMC-7721 cells were incubated with Oxa (10 μg/ml) for 24h. (B) HCC cell cycle were examined by flow cytometry (FCM) following PI staining. (C) Early apoptotic cells were detected by FCM following Annexin V-FITC/PI staining. (D) WB assay analyzed the protein expression of Cyclin D1, Bcl-2, Active Caspase3, and Cleaved Caspase Substrate. β-actin was used as a loading control. Representative results of three experiments were shown.