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. 2018 Oct 10;9(21):3962–3970. doi: 10.7150/jca.25909

Fig 2.

Fig 2

Oxaliplatin induced the expression of Cyr61, YAP and apoptosis related genes. (A) Differential expression genes of tumor tissues were screened between Oxa-treated and 5% GS-treated (control). (B-D) Bel-7404 and SMMC-7721 cells were incubated with Oxa (10 μg/ml) for 24h. (B) The expression levels of phosphorylated YAP (P-YAP), YAP, and Cyr61 were assayed by WB analysis. (C) Relative mRNA levels of YAP, CTGF, Cyr61, ANKRD1, and β-catenin were detected by quantitative RT-PCR analysis. Each bar represented means ±SD from three independent experiments. *, P<0.05, **, P<0.01 as analyzed by one-way ANOVA. (D) Oxaliplatin affected the intracellular location of YAP, which were examined by IF assay. (E) Early apoptotic cells were detected by FCM following Annexin V-FITC/PI staining. (F) WB assay detected the expression levels of P-YAP, YAP, Cyr61, Bcl2, and Cleaved Caspase Substrate in Bel-7404 and Bel-7404 YAPsh cells after treated with Oxa (10 μg/ml) for 24h. Representative results of three experiments were shown.