Fig 4.

Huaier enhanced Oxa-induced cell apoptosis by inhibiting the expression of YAP. (A-C) Bel-7404, Bel-7404 YAPsh, and SMMC-7721 cells were incubated with Oxa (10 μg/ml), HE (12mg/ml), or Oxa (10 μg/ml) combined with HE (12mg/ml) for 24h. (A) Cell viability was examined by CCK-8 assay in Bel-7404 and Bel-7404 YAPsh cells. *, P<0.05 as analyzed by Student t test. (B-C) WB assay was performed to detect the protein expression of P-YAP, YAP, Cyr61, CyclinD1, Bcl-2, and Cleaved Caspase Substrate. Representative results of three experiments were shown. (D) The expression level of P-YAP was normalized to the total YAP. Each bar represented means ± SD from three independent experiments. **, P<0.01 as analyzed by one-way ANOVA. (E) The underlying interaction mechanism between HE and Oxa in the regulation of YAP expression in HCC cell lines.