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. 2018 Oct 10;9(21):3912–3922. doi: 10.7150/jca.25900

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Downregulation of TOP2A inhibited cell proliferation by triggered cell cycle arrest at G2 phase in ccRCC cells. (A) The expression of TOP2A in transcriptional level (upper picture) and translation level (lower picture) by treating with siTOP2A-1, siTOP2A-2 and siTOP2A-3 in 786-O cell line. The proliferative capacity was measured by (B) MTT assay and (C) colony formation assays in 786-O cell line. (D) Flow cytometry analysis of TOP2A deficiency group and NC group in 786-O cell line. (E) Cell cycle related proteins such as Cyclin A1/2 and CDK1/2 were significantly decreased in the 786-O cell line in TOP2A knockdown group. (F) TOP2A deficiency strongly induced phosphorylated p53 in 786-O cell line.