Table 2.
The substrate specificity of the purified recombinant Bgl3Ba.
| Substrate | Glycosyl linkage | Specific activity (U/mg) | Relative activity (%)a |
|---|---|---|---|
| Aryl-glycosides | |||
| pNPG (2 mM) | β-Glucose | 222.8 ± 6.7 | 100.0 |
| Genistin (1%) | β-Glucose | 69.7 ± 0.2 | 31.3 |
| Daidzin (1%) | β-Glucose | 50.9 ± 0.2 | 22.8 |
| Amygdalin (1%) | - | 146.9 ± 0.1 | 65.9 |
| Oligosaccharides | |||
| Cellobiose (4 mM) | β-1,4-Glucose | 189.5 ± 1.8 | 100.0 |
| Cellotriose (1%) | β-1,4-Glucose | 185.0 ± 4.1 | 97.6 |
| Cellotetraose (1%) | β-1,4-Glucose | 94.1 ± 2.1 | 49.7 |
| Cellopentaose (1%) | β-1,4-Glucose | 85.8 ± 1.5 | 45.3 |
| Laminaritetraose (5 mM) | [β-d-Glc-1,3)]3-d-Glc | 173.0 ± 4.1 | 91.3 |
| Polysaccharides | |||
| Laminarin (1%) | β-1,3-Glucan | 25.7 ± 0.9 | 100.0 |
| Lichenan (0.5%) | 1,3:(1,4)2-β-d-Glucan | 25.6 ± 0.6 | 99.8 |
| Barley β-d-glucan (1%) | 1,3:1,4-β-d-Glucan | 7.2 ± 0.2 | 28.1 |
| Avicel (1%) | β-1,4-Glucose | 6.6 ± 0.2 | 25.5 |
| Xylan (1%) | β-1,4-Xylose | 4.1 ± 0.5 | 16.1 |
| CMC-Na (1%) | β-1,4-Glucose | 5.9 ± 0.4 | 23.0 |
aThe data are shown as mean ± SD (n = 3). The specific activities of Bgl3B towards pNPG, cellobiose, and laminarin are defined as 100% for the aryl-glycosides, oligosaccharides, and polysaccharides, respectively.