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. 2018 Oct 23;2018:4794690. doi: 10.1155/2018/4794690

Table 3.

Conversion of soybean isoflavone glycosides into free isoflavones by Bgl3B and the commercial glucosidase (G4511) from Sigma-Aldricha.

Enzyme Isoflavone glycosides (mg/ml) Free isoflavones (mg/ml)
Daidzin Genistin Glycitin Daidzein Genistein Glycitein
Control 45.2 ± 1.9 80.5 ± 4.7 7.3 ± 1.2 26.4 ± 2.8 15.3 ± 1.6 1.8 ± 1.1
Bgl3B 2.2 ± 0.8 19.3 ± 4.2 1.8 ± 3.2 42.2 ± 2.7 26.5 ± 1.8 3.7 ± 0.4
G4511 6.7 ± 0.3 28.8 ± 2.7 2.7 ± 0.8 36.8 ± 1.5 20.4 ± 0.7 3.0 ± 0.7

aResults are the mean of three replicates. The reaction systems without enzyme addition were treated as blank controls.