Table 3.
Conversion of soybean isoflavone glycosides into free isoflavones by Bgl3B and the commercial glucosidase (G4511) from Sigma-Aldricha.
Enzyme | Isoflavone glycosides (mg/ml) | Free isoflavones (mg/ml) | ||||
---|---|---|---|---|---|---|
Daidzin | Genistin | Glycitin | Daidzein | Genistein | Glycitein | |
Control | 45.2 ± 1.9 | 80.5 ± 4.7 | 7.3 ± 1.2 | 26.4 ± 2.8 | 15.3 ± 1.6 | 1.8 ± 1.1 |
Bgl3B | 2.2 ± 0.8 | 19.3 ± 4.2 | 1.8 ± 3.2 | 42.2 ± 2.7 | 26.5 ± 1.8 | 3.7 ± 0.4 |
G4511 | 6.7 ± 0.3 | 28.8 ± 2.7 | 2.7 ± 0.8 | 36.8 ± 1.5 | 20.4 ± 0.7 | 3.0 ± 0.7 |
aResults are the mean of three replicates. The reaction systems without enzyme addition were treated as blank controls.