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. 2018 Oct 19;29(11):2641–2657. doi: 10.1681/ASN.2018060573

Figure 2.

Figure 2.

Glomerular Shroom3 knockdown causes albuminuria in adult mice with FPE without podocyte loss. (A) Line graphs compares trend lines of serial ACRs in Podo-RTTA mice (Podo-TG) and nontransgenic littermates (Podo-NTG) upon DOX feeding, withdrawal and reinitiation. (B) ACR trend lines in CAGS-TG, CAGS-NTG, PAX8-TG, and PAX8-NTG animals. (C) Representative images of CAGS-NTG and -TG glomeruli with identification of podocytes (black arrowhead) for quantification by the fractionator/dissector technique. (D) Quantification of podocytes/glomerulus (n=8 mice per group; mean±SD). (E) Right to left: representative 40× immunofluorescence images for SYNPO, WT1, and merge (with DAPI), respectively (top row: CAGS NTG-animals, second row: CAGS-TG). (F) Podocyte quantification (n=4 mice per group) by WT1/DAPI-merge nuclei/glomerular profile, and (G) shows quantification of area SYNPO stain/glomerular profile (>30 glomeruli per kidney) (line/whiskers=mean/SD). (H) Right to left: representative electron microscopic images (3000×) of CAGS-NTG and -TG animals; left panel shows sample inlay magnified; Mean foot process width (five glomeruli per mouse) in representative NTG and TG animals were 267 and 441 nm, respectively. *P<0.05; **P<0.01; ***P<0.001.