Table 1.

Antibodies and reagents used for flow cytometry.

	Antigen	Clone/source of mAb	Detection/source
PANEL 1
Core	CD4	IL-A11/in house (ECACC)	Anti-IgG2a-PECy7/Southern Biotech
	CD13	CC81/Bio Rad	Directly conjugated to FITC
	CD172a	CC149/Bio Rad	Anti-IgG2b-Alexa647/Molecular Probes
	Flt3	n.a.	Anti-His-PE/Miltenyi
Phenotypic marker (Pm)	CD1	20.27/AdB Serotec	Anti-IgG1-biotin/Southern Biotech Streptavidin-BV421/BD Biosciences
	CD2	CC42/in house (ECACC)
	CD5	CC29/in house (ECACC)
	CD14	CAM36A/Kingfisher
	CD26	CC69/Bio Rad
	CD40	IL-A156/Bio Rad
	CD62L	Du-1-29/in house (ECACC)
	CD80	IL-A159/Kingfisher
	CD86	IL-A190A/Kingfisher
	CD205	IL-A114/Bio Rad
	CD163	LND68A/Kingfisher
	BoLA-DRA	VPM54/in house (ECACC)
PANEL 2
Core	CD4	CACT83B/Kingfisher	Anti-IgM-Alexa647/Southern Biotech
	CD13	CC81/AdB Serotec	Anti-IgG1-biotin/Southern Biotech Streptavidin-BV421/BD Biosciences
	CD172a	CC149/Bio Rad	Anti-IgG2b-Alexa488/Molecular Probes
	Flt3	n.a.	Anti-His-PE/Miltenyi
Pm	CD16	KD1/Bio Rad	Anti-IgG2a-PECy7/Southern Biotech
PANEL 3
Core	CD4	IL-A11/in house (ECACC)	Anti-IgG2a-PECy7/Southern Biotech
	CD13	CC81/Bio Rad	Directly conjugated to FITC
	CD172a	DH59B/Kingfisher	Anti-IgG1-biotin/Southern Biotech Streptavidin-BV421/BD Biosciences
	Flt3	n.a.	Anti-His-PE/Miltenyi
Pm	CD11b	MM10A/Kingfisher	Anti-IgG2b-Alexa647/Molecular Probes
PANEL 4
Core	CD4	IL-A11/in house (ECACC)	Anti-IgG2a-PECy7/Southern Biotech
	CD13	CC81/Bio Rad	FITC directly conjugated
	CD172a	DH59B/Kingfisher	Anti-IgG1-biotin/Southern Biotech Streptavidin-BV421/BD Biosciences
	Flt3	n.a.	Anti-His-PE/Miltenyi
Pm	CD11c	BAQ153A/Kingfisher	Anti-IgM-Alexa647/Southern Biotech
	CD71	IL-A77A/WSU
	CADM1	3E1/MBL	Directly conjugated to Alexa647