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. Author manuscript; available in PMC: 2018 Nov 6.
Published in final edited form as: Cell Rep. 2018 Sep 18;24(12):3251–3261. doi: 10.1016/j.celrep.2018.08.065

Figure 4. HLTF Counteracts Replication Stress in FANCJ-KO Cells.

Figure 4.

(A) Western blot analysis with the indicated Abs of lysates from control and FANCJ-KO U2OS cells and when expressing shRNA against HLTF or NSC.

(B) Cell survival assays with FANCJ-KO and control U2OS cells expressing shRNA against HLTF or NSC under increasing concentrations of MMC or HU. Data represent the mean percent ± SD of survival from three independent experiments.

(C) γ-H2AX/EdU ratio in FANCJ-KO and control U2OS cells expressing shRNA against HLTF or NSC following treatment with HU. At least 200 cells were measured for each. The assay was completed in triplicate for each shRNA.

(D) Quantification of the percentage of comet tail DNA in FANCJ-KO and control 293T cells expressing shRNA against HLTF or NSC following treatment with HU, and images of comet tail formation. 100 cells were measured for each. Statistical analysis according to two-tailed Mann-Whitney test; ****p < 0.0001; *p < 0.05.