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. Author manuscript; available in PMC: 2018 Nov 6.
Published in final edited form as: Cell Rep. 2018 Sep 18;24(12):3251–3261. doi: 10.1016/j.celrep.2018.08.065

Figure 5. FANCJ Contributes to Unrestrained Replication and S1 Nuclease Sensitivity in HLTF-Depleted Cells.

Figure 5.

(A) Western blot analysis with the indicated Abs of lysates from HCT116 cells expressing shRNA against FANCJ, HLTF, or NSC.

(B) Schematic, representative images, and quantification of CldU tract length during HU treatment.

(C) Schematic and quantification of the CldU/IdU ratio in the indicated shRNA-expressing HCT116 cells with or without S1 nuclease incubation. Each dot represents one fiber; at least 200 fibers are quantified from two independent experiments. Red bars represent the median. Statistical analysis according to two-tailed Mann-Whitney test; ****p < 0.0001.

(D) Model of opposing roles of FANCJ and HLTF at forks.