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. 2018 Nov 6;17(Suppl 2):156. doi: 10.1186/s12938-018-0579-0

Table 2.

PCR protocol used in the experiments

PCR process Temperature (°C) Time duration
1 cycle 95 3 min
40 cycles 95 10 s
60 30 s
72 30 s
1 cycle 95 10 s
50 30 s