Fig. 1.

PRRSV infection causes reduction of MARC-145 cells number. aThe standard curve of OD450nm and cell numbers. 1000, 2000, 4000, 8000, 16000, or 32000 cells were seeded in 96-well plate, and CCK-8 solution was added. The OD450nm were measured at 2 h after adding CCK-8 solution. Data shown are mean ± SEM from six repeated experiments. b Different MOI viral infection reduces MARC-145 cells number. MARC-145 cells were infected with PRRSV SD16 at 0.01, 0.1, 1, and 5 MOI. At 6, 12, 24, 36, 48, and 72 h after infection, CCK-8 solution was added, and the numbers of cells were calculated according to the standard curve. c PRRSV infection gives rise to typical CPE in infected MARC-145 cells (× 100)