Table 2.
Experimental validation of variation in miRNA expression
| Experimental condition | MicroRNA | Relative expression | Std Error | 95% C.I. | P(H1) | Result |
|---|---|---|---|---|---|---|
| SfC (Rice/Corn) | miR-31 (Ref) | 1 | ||||
| miR-34 | 1.763 | (1.080–3.062) | 1.003–3.513 | 0.004 | UP | |
| miR-190 | 1.549 | (1.320–1825) | 1.235–2.032 | 0.001 | UP | |
| SfR (Rice/Corn) | miR-31 (Ref) | 1 | ||||
| miR-190 | 1.218 | 0.671–1.520 | 0.900–1.669 | 0.05 | UP | |
| tca-miR375* | 5.802 | (3.115–11,105) | 1.511–14.148 | 0.002 | UP | |
| On Corn (SfR/SfC) | miR-31(Ref) | 1 | ||||
| miR-34 | 1.868 | 1.395–2.466 | 1.282–2.811 | 0.003 | UP | |
| dme-miR-275* | 0.965 | 0.821–1.121 | 0.726–1.251 | 0.587 | ||
| On Rice (SfR/SfC) | miR-31(Ref) | 1 | ||||
| dme-miR-275* | 1.434 | 1.236–1.545 | 1.194–1.907 | 0.001 | UP |
The relative expression of miR genes depending on the host-plant (on rice compared to corn) in either the C or the R strain, or depending on the genetic background (SfR compared to SfC) was calculated according to [57]
P(H1) Probability of alternate hypothesis that difference between sample and control groups is due only to chance. The miRs with a star correspond to * Novel miRs having conserved seed region only