Table 3.

List of target genes of known miRs and their differential expression

MiR				Putative target genes
Name	Sf strain	Regulated on	Log2FC	aPredicted Targets	Down or up regulated targets	Down-regulated	bAmong down and up- regulated genes	Log2FC
miR-34			2.32	321	38	28	take-out GSSPFG00021718001-RA cuticular proteins	−4.22
							GSSPFG00000626001-RA	−3.24
							GSSPFG00013845001-RA	−1.6
							GSSPFG00010502001-RA subunit DSS1 of 26S proteasome	−1.21
							GSSPFG00027010001-RA	−2.14
miR-190			2.07	479	46	25	take-out GSSPFG00021718001-RA cuticular protein	−4.22
							GSSPFG00005155001-RA acyl-CoA desaturase	−3.54
							GSSPFG00006314001-RA osiris 9A	−6.54
							GSSPFG00012033001-RA	−4.71
miR-1a-5p			1.33	524	46	18	Serine proteinase GSSPFG00014426001-RA	−3.38
miR-998			− 3.33	229	31	10	sodium- and chloride-dependent glycine transporter 1-like GSSPFG00018036001-RA facilitated trehalose transporter Tret1	2.61
							GSSPFG00023681001-RA Spermine oxidase	1.31
							GSSPFG00025432001-RA	3.02
miR-278			−2.50	91	8	4	integrin alpha-PS2 GSSPFG00025987001	1.15
miR-263a-5p			−2.18	436	56	27	sodium- and chloride-dependent glycine transporter 1-like GSSPFG00018036001-RA facilitated trehalose transporter Tret1	2.61
							GSSPFG00023681001-RA Spermine oxidase	1.31
							GSSPFG00025432001-RA	3.02
miR-10-5p			−1.96	151	20	12	sodium- and chloride-dependent glycine transporter 1-like GSSPFG00018036001-RA facilitated trehalose transporter Tret1	2.6
							GSSPFG00012081001-RA	1.61
Name	Host-plant	Regulated in	Log2FC	aPredicted Targets	Down and up- regulated targets	Down-regulated	bAmong down and up- regulated genes	Log2FC
miR-34			1.72	321	30	18	transcription factor ap-2 GSSPFG00001565001-RA lysine-specific demethylase 2B	−1.3
							GSSPFG00019875001-RA graves disease carrier protein	−1.24
							GSSPFG00028641001-RA	−1.08
miR-263a-5p			−2.69	436	43	26	unknown secreted protein [Papilio polytes] GSSPFG00016698001-RA cuticular protein RR-1 motif 26	1.96
							GSSPFG00021712001-RA	1.54
miR-10-5p			−2.48	151	25	7	proton-coupled folate transporter GSSPFG00020391001-RA facilitated trehalose transporter Tret1-like	1.11
							GSSPFG00012081001-RA	1.02
miR-278-5p			−1.36	91	7	3	zinc finger protein 347 GSSPFG00017383001-RA	0.76

Clipart for rice is available by Google [Apache License 2.0 (http://www.apache.org/licenses/LICENSE-2.0)], via Wikimedia Commons, https://upload.wikimedia.org/wikipedia/commons/5/58/Emoji_u1f33e.svg and for corn by Spedona - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=5310440

^aTarget genes were predicted using Targetscan software. ^bThe predictions that had been confirmed by at least one other software among miRanda, Rna22 or miRmap and that had been shown to be expressed and regulated in parallel Reciprocal Transplant experiments [7] are listed with the corresponding foldchange. The complete target gene list figure in Additional file 10: Excel file S5 and Additional file 11: Excel file S6. Pictures of S. frugiperda larvae on plants were taken by Marion Orsucci who gave the written permission to use and adapt them