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. 2018 Nov 1;11:7613–7624. doi: 10.2147/OTT.S179866

Figure 2.

Figure 2

SIRT6 overexpression affected viability of NPC cells.

Notes: (A, B) Western blotting and (C, D) qRT-PCR were performed to evaluate SIRT6 expression in 5-8 F and CNE1 cells following infection with adenovirus-SIRT6 (AD-SIRT6) or AD-NC. (E, F) MTT assay was used to examine the proliferation of 5-8 F and CNE1 cells 12–72 hours post-culture. (G, H) Soft agar colony formation assay for the 5-8 F and CNE1 cells infected with AD-SIRT6, AD-NC, and that for uninfected cells. (I, J) The number of colonies formed in each group. Data are shown as mean ± SD. **P<0.01, ***P<0.001.

Abbreviations: AD, adenovirus; NC, negative control; NPC, nasopharyngeal carcinoma.