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. 2018 Nov 1;11:7715–7724. doi: 10.2147/OTT.S178597

Figure 2.

Figure 2

Knockdown of PCAT6 inhibits NSCLC cell growth.

Notes: (A) si-PCAT6 or si-Con was transfected into A549 and H1975 cells. The levels of PCAT6 were determined by qRT-PCR assay. **P<0.01 compared with control, Student’s t-test. (B, C) The cell proliferation of A549 and H1975 cells that was transfected with si-PCAT6 was determined by MTT assay, one-way ANOVA. (D, E) The colony formation analysis of si-Con or si-PCAT6 transfected A549 and H1975 cells. **P<0.01 compared with si-Con, Student’s t-test. (F) Knockdown of PCAT6 significantly inhibits A549 cell growth in xenograft model, one-way ANOVA. **P<0.01 compared with si-Con. (G) Tumor weight, **P<0.05 compared with si-Con, Student’s t-test. (H) The levels of Ki-67 in tumor that was formed by si-PCAT6 or si-Con-transfected A549 cells were detected by immunohistochemical staining. **P<0.01 compared with si-Con, Student’s t-test.

Abbreviations: NSCLC, non-small-cell lung carcinoma; PCAT6, prostate cancer-associated transcript 6; qRT-PCR, quantitative real-time polymerase chain reaction.