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Sequences of primers and probes used in the LNA kdr assay with 5′ and 3′ modifications indicated
| Name | 5′ Fluorescence modification | Sequence | 3′ Quencher modification |
|---|---|---|---|
| VGSC-F | CGTGTGCTATGCGGAGAATG | ||
| VGSC-R | CGATCTTGGTCCATGTTAATTTGC | ||
| Kdr-Leu | HEX | A+CGA+C+T+AAAT+TTC+C | IBFQ |
| Kdr-Phe | 6-FAM | A+CGA+C+A+AAAT+TTC+C | IBFQ |
| Kdr-Ser | Cy5 | +CGA+C+T+GAAT+TTC+C | IBRQ |
+, preceding a base indicates it is a LNA nucleotide
