Table 1.

Questionnaire pertained to the matter of biosecurity, reproductive health of the livestock, maintenance of cattle sheds and knowledge of zoonoses

S.N.	Issue addressed	Relevance to Brucellosis
1.	Consumption of raw milk and Application of raw milk on cracked lips	Infected buffalo secrete large amounts of Brucella in their milk.
2.	Assisting animal birth, application of intrauterine medication post abortion, disposing aborted foetus and placenta with naked hands.	Uterine fluid, Placental membranes, aborted foetus of infected buffalo during parturition or abortion are rich sources of Brucella.
3.	History of abortion and retained placenta	The seroprevalence of brucellosis is found to be significantly higher in animals with a history of abortion and retained placenta.
4.	Knowledge about Brucellosis or any other zoonoses and S19 vaccine	Knowledge about a disease makes the high risk population cautious and thus prevents the spread. Vaccination of young animals is known to reduce burden of disease.
5.	Rearing small ruminants with large ruminants	Infectious diseases spillover from small ruminants to large ruminants and cause huge economic loss.
6.	Sleeping in cattle sheds	Close contact with buffalo is a risk factor identified for human Brucellosis.
7.	Blood testing before sale and purchase of cattle	Diagnosis of brucellosis may curb the sale of non-productive buffalo and curb the spread of disease.
8.	Separation of sick animals	Intermingling of sick buffalo or domesticated cattle like Bos sp. with healthy buffalo may facilitate the transmission of brucellosis to susceptible cattle.
9.	Use of disinfectant to clean the cattle shed	Disinfectants lyse the gram negative bacteria and thus remove infection from the environment of the cattle shed.