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. 2018 Nov 6;18:1068. doi: 10.1186/s12885-018-4903-7

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Immunohistochemical staining of GPNMB in human tissue microarray. 1 mm core were used from each patient’s FFPE Block on the TMAs. a Positive GPNMB staining of brown color (DAB) represented in membrane in normal colon biopsies, some staining was also noted in the stroma. b Moderate cytoplasmic membrane staining marked in biopsy specimens from advanced adenoma. c CRC patients showed absence of GPNMB staining in the malignant cells. Statistical analysis was performed using the chi-square test