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. 2018 Nov 6;8:57. doi: 10.1186/s13578-018-0256-9

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — CITED1 promotes proliferation of PTC cell lines. a Overexpression of CITED1 was confirmed in the studied cells (TPC-1 and BCPAP) by western blotting analysis. α-tubulin was used as the loading control. b MTT assays were conducted to examine the viability of the indicated cell lines. c Representative images (upper panel) and relative quantification (lower panel) of the colony formation assay results. d Proportions of cells in distinct cell-cycle phases in the indicated cell lines were determined by flow cytometry. Overexpression of CITED1 decreased the proprotion of cells in G1 phase. e Representative micrographs (left panel) and quantification (right panel) of the EdU incorporation assay results. For b, c, and e, the results derived from three independent experiments and are expressed as the mean ± SD. *P < 0.05