Fig. 5.
Knockdown of CITED1 upregulates the expression of the CKIs p21 and p27. a Western blotting analysis was performed to detect the expression levels of the cell cycle regulators cyclin A2, cyclin B1, cyclin D1, cyclin D2, cyclin D3, cyclin E1, cyclin E2, CDK2, CDK4, CDK6, p21Cip1, and p27Kip1 in the indicated cells. α-tubulin was used as the loading control. b Silencing of CITED1 in the studied cells significantly inhibited the phosphorylation of pRb at the Ser608 residue. α-tubulin served as the sample loading control. c Silencing of CITED1 attenuated E2F transcriptional activity according to the E2F-luc reporter assay. The results are derived from three independent experiments and are expressed as the mean ± SD. *P < 0.05