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CITED1 overexpression inhibits the levels of p21 and p27. a Western blotting analysis was performed to detect the expression levels of p21 and p27, as well as the phosphorylation of pRb. α-tubulin was used as the loading control. b Transcriptional activity of E2F was analyzed by luciferase reporter assays. pRL-TK was used to normalize the luciferase activity. For b, data from three independent experiments are shown as the mean ± SD. *P < 0.05
