Fig. 5.

Expression patterns of flavonoid-related AS transcripts. a Heat map showing the FPKM of non-AS and AS transcripts in eight tissues (B, bud; YL, young leaf; SML, summer mature leaf; WOL, winter old leaf; S, stem; R, root; FL, flower; FR, fruit). Red and blue colors represent high and low transcript abundance, respectively. b Read coverage graphs of TEA003146.1 and TEA012130.1 derived from eight tissues viewed in IGV and validated by semi-quantitative RT-PCR. The red dotted boxes indicate the positions of alternative splicing. The RT-PCR bands are shown in the gel images beneath the graphs. The CsGAPDH gene was used as the internal control