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. Author manuscript; available in PMC: 2018 Nov 6.
Published in final edited form as: Biochem J. 2011 Oct 1;439(1):57–65. doi: 10.1042/BJ20101700

Figure 2. An acidic DID region contributes to mDia2 auto-regulation.

Figure 2

(A) Conservation of an acidic region within the mDia2 DID α5B helix. (B) 10T1/2 cells were transfected with wild-type (Wt) or E377A/D378A (377/8A) mDia2 along with luciferase constructs driven by the SM22, SM α-actin or c-Fos promoter. *P < 0.05 compared with wild-type. Ev, empty vector. (C) HeLa cells were transfected with E377A/D378A mDia2, serum-starved for 24 h, and stained with phalloidin. (D) FLAG–mDia2 DAD was immunoprecipitated (IP) from Cos-7 cells co-expressing the indicated GFP-tagged N-terminal mDia2 fragment. Immunoprecipitants were probed with anti-GFP or anti-FLAG antibodies. IB, immunoblot. (E) 10T1/2 cells were transfected with the SM22 luciferase promoter, mDia2 F1F2-C, and increasing amounts of the indicated N-terminal mDia2 fragment. Luciferase activities were normalized to that obtained upon expression of mDia2F1F2-C alone set to 100 %. *P < 0.05 compared with control. FAK, focal adhesion kinase.