Figure 3. . Evaluation of DNA transfection efficiency of PEGylated-poly(lactic-co-glycolic acid)/polyethyleneimine nanoparticles in MDA-MB-231 triple negative breast cancer cells by bioluminescence imaging.
pcDNA-Fluc-eGFP DNA (1 μg) transfection using different concentration of PEGylated poly(lactic-co-glycolic acid)/polyethyleneimine nanoparticles in MDA-MB-231 cells and transfection analysis by bioluminescence imaging. (A) Graph showing the quantitative bioluminescence signal measured (p/s/cm2/sr) from cells treated with respective transfection condition (*p < 0.001, **p < 0.01, ***p < 0.05). Top: Bioluminescence images of cells acquired by optical CCD camera. (B) Immunoblot analysis of TK and TK-NTR proteins in MD-MB-231 cells transfected with respective plasmid DNA using poly(lactic-co-glycolic acid)/polyethyleneimine nanoparticles.