Figure 5. . Therapeutic evaluation of thymidine kinase-nitroreductase gene directed enzyme prodrug therapy system in MDA-MB-231 cells transfected using PEGylated poly(lactic-co-glycolic acid)/polyethyleneimine nanoparticles.

The cells transfected with 1 μg of pcDNA-thymidine kinase-nitroreductase plasmid DNA complexed with different concentration (0–41.25 μg) of PEGylated-poly(lactic-co-glycolic acid)/polyethyleneimine nanoparticles for 24 h was evaluated for gene directed enzyme prodrug therapy mediated apoptotic induction at different time points (24, 48 and 78 h) post-treatment with prodrug ganciclovir and CB1954 combination by propidium iodide staining based FACS analysis. Figure showing the histogram of cells analyzed at each condition and marked for dead and live cell populations.