Table 1. . Synthesis, optimization and characterization of PEGylated poly(lactic-co-glycolic acid)/polyethyleneimine nanoparticles.

Sl No	PLGA-PEG Weight (mg)	PEI weight (mg)	Method	Surfactant	Organic solvent	Size	PDI	(ζ)-Potential (mV)
1	10	7*	W/O/W	1% PVA	DCM	560.3	0.530	+62.1
2	‘’	2*	‘’	‘’	‘’	537.6	0.420	+60.5
3	‘’	1*	‘’	‘’	‘’	161.3	0.311	+44.2
4	‘’	2*	EDE	2% PVA	Ethyl acetate	245.6	0.286	+52.5
5	‘’	2*	‘’	No surfactants	‘’	277.7	0.447	+40.2
6	‘’	1*	W/O/W	‘’	DCM	426.9	0.348	+66.6
7	‘’	2‡	‘’	‘’	‘’	436.7	0.461	+63.2
8	‘’	2‡	‘’	1% PVA	‘’	204.5	0.203	+35.3
9§	‘’	2‡	‘’	‘’	‘’	211.7	0.194	+25.5

*Linear PEI was added in W1 phase;

^‡Linear PEI was added in W2 phase;

^§10 nmol of anti-miR-21 (10% Cy5-cojugated anti-miR-21) loaded to the NPs with 50.4% encapsulation efficiency.

DCM: Dichloromethane; EDE: Emulsion-diffusion-evaporation; NP: Nanoparticle; PDI: Polydispersity index; PEG: Polyethylene glycol; PEI: Polyethyleneimine nanoparticle; PLGA: Poly(lactic-co-glycolic acid); PVA: Polyvinyl alcohol; W/O/W: Water-oil-water.