MDM following treatment with (A) URMC-099, (B) rapamycin, (C) metformin, (D) DMC, (E) HBC and (F) clonidine at various concentrations were analyzed by the MTT assay. MDMs were incubated with MTT (5 mg/ml) for 30 min at 37°C. Depending on viability, MTT was reduced to insoluble formazan crystals which was solubilized into a purple colored solution by DMSO. Results are shown as percentage of cell viability as compared with untreated MDM. Data are represented as mean ± SD for n = 4 samples/group.
DMC: Desmethylclomipramine; HBC: 2-hydroxy-β-cyclodextrin; MDM: Monocyte-derived macrophage; MTT: (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltriazolium bromide; SD: Standard deviation.