Figure 4. . Autophagosomal retention of atazanavir.
Human MDM were treated with CF633 labeled nanoATV (red) for 16 h followed by the panel of autophagy inducers for 14 days. Cells were transfected with a LC3B-GFP construct (green), stained with DAPI (blue) and analyzed by confocal microscopy. Scale bar: 20 μm.
ATV: Atazanavir; MDM: Monocyte-derived macrophage.