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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Biochim Biophys Acta Mol Basis Dis. 2018 Aug 8;1864(10):3527–3536. doi: 10.1016/j.bbadis.2018.08.007

Figure 2.

Figure 2.

ROS are required for TRPC6 upregulation by suPAR in mouse podocytes. (a) Increases in ROS evoked by suPAR are prevented in cells concurrently treated with 100 μM TEMPOL, a membrane-permeable ROS quencher. (b) Concurrent exposure to 100 μM TEMPOL also prevents increase in steady-state cell surface expression of TRPC6 evoked by suPAR, as measured by cell-surface biotinylation assay. (c) Examples of whole-cell recordings show that membrane stretch-evoked cationic currents (previously shown to be mediated by TRPC6) are markedly increased in cells treated with suPAR (10 ng/ml for 24 hr). However, this did not occur in cells concurrently exposed to suPAR and TEMPOL or in cells exposed to TEMPOL alone for 24 hr. (d) Bar graphs showing mean fold increases in current (measured at +80 mV) evoked by membrane stretch depending on previous 24 hr of treatment.